| S.N | Vertebrates | Invertebrates |
| 1. | They have backbone or vertebral column. | They do not have backbone or vertebral column. |
| 2. | They are the most organized animals on the earth. | They are not organized as vertebrates. |
| 3. | They consist of single phylum. | They consist of eight phylum. |
| 4. | They have internal skeleton. | Internal skeleton absent. |
| 5. | They have two pairs of limbs. | They have three or more pairs of limbs. |
| 6. | Heart is on the ventral side of the body. | Heart if present, is on the dorsal side of the body. |
| 7. | Nerve cord is dorsal and hollow. | Nerve cord is ventral and solid. |
| 8. | Haemoglobin in red blood cells. | Haemoglobin, if present, dissolved. |
| 9. | A tail is usually present. | Tail is absent. |
| 10. | Less than 10% of animals on the earth. | Over 90% of animals on the earth. |
| 11. | They have notochord during embryonic period. | They do not have notochord. |
| 12. | They are large sized animals. | They are small and slowly moving animals. |
| 13. | Pharynx has pharyngeal clefts that help in breathing. | They do not have pharyngeal clefts. |
| 14. | They have a closed circulatory system. | Most have an open circulatory system. |
| 15. | They have hepatic portal system. | They do not have such system. |
| 16. | Skin consists of epidermis and dermis layer. | Skin consists of a single layer. |
| 17. | Eyes are an outgrowth from the brain. | Eyes are an outgrowth from the skin. |
| 18. | Do not have compound eyes. | They have compound eyes. |
| 19. | They are mostly unisexual. | They may be unisexual or bisexual (hermaphrodite). |

Image source: thebiologynotes
References:
i)https://byjus.com/biology/invertebrate-and-vertebrate-difference/
ii) https://www.vedantu.com/biology/difference-between-vertebrates-and-invertebrates