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Steps and procedure of polymerase chain reaction (PCR)

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Steps and procedure of polymerase chain reaction (PCR)

Steps of PCR Many cycles of DNA synthesis are carried out in PCR. These cycles are staged by controlling the temperature that reaction takes place. The various steps are: 1. Denaturation of DNA The double stranded DNA should be heated to 940– 960C to break a part the hydrogen bonds forming single strands. Denaturing is … Read more

Polymerase chain reaction (PCR) and its principle

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Polymerase chain reaction (PCR) and its principle

Introduction of PCR Polymerase chain reaction (PCR) is one of the significant advances in DNA based technology. It is a rapid, sensitive, inexpensive and simple means of producing relatively large number of copies of DNA molecules from minute copies of source DNA (bacterial, viral, plant and animal). It does not depend upon the quality of … Read more