Jaundice and its types

Jaundice is a clinical condition characterized by the yellow color of the white of the eyes (sclera) and skin. It is caused by the deposition of bilirubin due to its elevated levels in the serum. Hyper-bilirubinemia is often used to represent the increased concentration of serum bilirubin. The normal serum total bilirubin concentration is in … Read more

ELISA and its type

It is the abbreviated form of Enzyme-linked immunosorbent assay or EIA. It is similar in principle to Radio immune assay (RIA) but instead of radioactive substance it uses an enzyme. Chromogenic substrate is generated by reacting an enzyme conjugated with the antibody and a colorless substrate. Chromogenic substrate is a colored reaction product. Various enzymes … Read more

Principle and procedure of Southern blotting

Principle and procedure of Southern blotting

Introduction It is a method developed by E.M Southern (1975). It combines the use of restriction endonuclease, electrophoresis, and DNA probes for analyzing the related genes in a DNA restriction fragment. It is used for various purposes. They are: To detect sequence homology between two DNA molecules without determining the exact base sequence of the … Read more