DNA template

Components of polymerase chain reaction

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Components of polymerase chain reaction

There are various components of PCR for conducting this process. They are DNA template, primers, Taq polymerase, deoxyribonucleoside triphosphates, PCR buffer, dimethyl sulfoxide and thermocycler.   1. DNA template It contains the region of the DNA fragment to be amplified by the PCR process. Successful amplification of the region of interest is dependent upon the … Read more

Steps and procedure of polymerase chain reaction (PCR)

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Steps and procedure of polymerase chain reaction (PCR)

Steps of PCR Many cycles of DNA synthesis are carried out in PCR. These cycles are staged by controlling the temperature that reaction takes place. The various steps are: 1. Denaturation of DNA The double stranded DNA should be heated to 940– 960C to break a part the hydrogen bonds forming single strands. Denaturing is … Read more