primers

Components of polymerase chain reaction

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Components of polymerase chain reaction

There are various components of PCR for conducting this process. They are DNA template, primers, Taq polymerase, deoxyribonucleoside triphosphates, PCR buffer, dimethyl sulfoxide and thermocycler.   1. DNA template It contains the region of the DNA fragment to be amplified by the PCR process. Successful amplification of the region of interest is dependent upon the … Read more

Polymerase chain reaction (PCR) and its principle

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Polymerase chain reaction (PCR) and its principle

Introduction of PCR Polymerase chain reaction (PCR) is one of the significant advances in DNA based technology. It is a rapid, sensitive, inexpensive and simple means of producing relatively large number of copies of DNA molecules from minute copies of source DNA (bacterial, viral, plant and animal). It does not depend upon the quality of … Read more