Category: Microbiology

Introduction Sterilization is an essential stage which is used in the processing of any product. These products are destined for parenteral administration, or for contact...

Introduction of chemical mutagens It is a mutation agent which is a chemical substance that mimic nitrogen base in normal DNA without coupling during DNA...

Introduction It is called as soluble RNA because of its small size made up of 75-95 nucleotides and sedimentation coefficient of mature eukaryotic t-RNA is...

Introduction Specialized transduction can be distinguished from generalized transduction by two properties. Only bacterial genes close to the attachment site of pro-phage can be transduced...

Introduction Immunodeficiency diseases are condition where the defense mechanism of the body is impaired lading to repeated microbial infections of varying severity and sometimes enhanced...

Introduction It is a method developed by E.M Southern (1975). It combines the use of restriction endonuclease, electrophoresis, and DNA probes for analyzing the related...

There are various components of PCR for conducting this process. They are DNA template, primers, Taq polymerase, deoxyribonucleoside triphosphates, PCR buffer, dimethyl sulfoxide and thermocycler....

Polymerase chain reaction has various advantages. Some of the advantages of it are as follows. 1. Cloning genes It is used to amplify the gene...

Steps of PCR Many cycles of DNA synthesis are carried out in PCR. These cycles are staged by controlling the temperature that reaction takes place....

Introduction of PCR Polymerase chain reaction (PCR) is one of the significant advances in DNA based technology. It is a rapid, sensitive, inexpensive and simple...